all MUGEN mice

NALP3


General Information
MUGEN ID
M153001
Common Line Name
NALP3
Research Applications Type
MODEL OF HUMAN DISEASE
Contact
Tschopp Jurg
Institution
UNIL
Research Applications Comments
Mouse model to study inflammatory diseases.

Availability
Repository Available Background State Type
UNIL BalB/c live mouse mutant strain
UNIL C57BL/6 live mouse mutant strain

Genetic Background
DNA Origin Targeted Background Host Background Backcrossing Strain Backcrosses
neo: bacterial C57BL/6J C57BL/6J BalB/c-Hsd 08

Strain Information
Designation
B6-Nlrp3tm1Tsc/Siec

Allele & Mutations
Name Symbol Mutation(s)
targeted mutation 1, Jurg Tschopp Nlrp3tm1Tsc insertion, targeted mutation

Gene(s)
Name Comment Chromosome
NLR family, pyrin domain containing 3 not provided yet 11

Handling & Genotyping Instructions
Name Type
Docs
NALP3 mice PCR directions.docFile

Mammalian Phenotype Ontologies
 
MP Term: homeostasis/metabolism phenotype>abnormal homeostasis>other metabolic defect>abnormal response/metabolism to endogenous compounds
PATO Logical Definition: 
 
MP Term: immune system phenotype>abnormal immune system physiology>abnormal inflammatory response>abnormal inflammatory mediator physiology>abnormal cytokine physiology>abnormal interleukin physiology>abnormal IL-1 physiology
PATO Logical Definition:  functionality qualifier abnormal inheres_in IL-1
 

References
Name Type Comment
Martinon et al., 2006, Nature, 440(7081):237-41 Webblink Gout-associated uric acid crystals activate the NALP3 inflammasome.

Author's Comments
 
EGFP cessette inserted in frame with ATG of exon 2 to disrupt the gene. A PGK-neo cassette flanked by 2 loxP sites was also inserted in intron 2. The neo cassette was deleted by backcrossing the mice with a Cre-expressing deleter strain.