all MUGEN mice

NEMOFL


General Information
MUGEN ID
M193026
Common Line Name
NEMOFL
Research Applications Type
MODEL OF IMMUNE PROCESSES
Contact
Pasparakis Manolis
Institution
CGN
Research Applications Comments
Inflammation/Immune Modulation

Availability
Repository Available Background State Type
CGN C57BL/6 live mouse mutant strain

Genetic Background
DNA Origin Targeted Background Host Background Backcrossing Strain Backcrosses
Nemo, mouse; neo, bacterial Bruce 4 of C57B/6 origin CB20 or BALB/c C57BL/6 >=10

Strain Information
Designation
B6-Ikbkgtm1.1Mpa

Allele & Mutations
Name Symbol Mutation(s)
targeted mutation 1.1, Manolis Pasparakis Ikbkgtm1.1Mpa targeted mutation

Gene(s)
Name Comment Chromosome
inhibitor of kappaB kinase gamma not provided yet X

Handling & Genotyping Instructions
Name Type

Mammalian Phenotype Ontologies
 
MP Term: lethality-embryonic/perinatal>embryonic lethality
PATO Logical Definition: 
 
MP Term: skin/coat/nails phenotype>abnormal skin condition/ morphology>abnormal skin condition>skin edema
PATO Logical Definition:  edematous inheres_in skin
 

References
Name Type Comment
Schmidt-Supprian et al., 2000 Mol Cell 5(6):981-92 Webblink NEMO/IKK gamma-deficient mice model incontinentia pigmenti.

Author's Comments
 
Exon II of NEMO was flanked by loxP sites. Removal of exon II through Cre-mediated excision deletes amino acids 64 to 134 of NEMO and introduces a frameshift to the downstream exons resulting in a null NEMO allele.