all MUGEN mice

Cst3-/-


General Information
MUGEN ID
M215009
Common Line Name
Cst3-/-
Research Applications Type
MODEL OF IMMUNE PROCESSES
Contact
Holmdahl Rikard
Institution
ULUND
Research Applications Comments
Cystatin C knock-out, affects CIA severity

Availability
Repository Available Background State Type
ULUND C57BL/10.H2q live mouse congenic strain

Genetic Background
DNA Origin Targeted Background Host Background Backcrossing Strain Backcrosses
Cystatin C, human & mouse (129/Sv); PGK-neo cassette, pKJ1 plasmid 129/Sv C57BL/DBA F1 C57BL/6J >=10

Strain Information
Designation
B6;129-Cst3tm1Karl

Allele & Mutations
Name Symbol Mutation(s)
targeted mutation 1, S. Karlsson Cst3tm1Karl targeted mutation

Gene(s)
Name Comment Chromosome
cystatin C not provided yet 2

Handling & Genotyping Instructions
Name Type

Mammalian Phenotype Ontologies
 
MP Term: normal phenotype>no abnormal phenotype detected
PATO Logical Definition: 
 

References
Name Type Comment
Huh et al., 1999 Mol Pathol 52(6):332-40 Webblink Decreased metastatic spread in mice homozygous for a null allele of the cystatin C protease inhibitor gene.

Author's Comments
 
After intravenous injection of tumour cells, the CysC−/− mice had significantly fewer lung metastases. Cystatin C concentrations in vivomight influence metastasis in some tissues.