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BQ.JDTA


General Information
MUGEN ID
M217010
Common Line Name
BQ.JDTA
Research Applications Type
MODEL OF IMMUNE PROCESSES
Contact
Holmdahl Rikard
Institution
ULUND
Research Applications Comments
Replacement of Igj exon 1 with DTA chain gene and a floxed neo cassette. The floxed neo cassette can removed by transient expression of cre recombinase in correctly targeted ES cells, thus allowing transcription from the DTA gene.

Availability
Repository Available Background State Type
ULUND C57BL/10.H2q cryopreserved embryos unknown

Genetic Background
DNA Origin Targeted Background Host Background Backcrossing Strain Backcrosses
J chain gene locus, mouse; DTA, pUC plasmid; neo, bacterial 129 ES (E14) 129P2/OlaHsd C57BL/10.H2Q >=10

Strain Information
Designation
B10;129-Igjtm1Tl

Allele & Mutations
Name Symbol Mutation(s)
targeted mutation 1, Tomas Leanderson Igjtm1Tl targeted mutation

Gene(s)
Name Comment Chromosome
immunoglobulin joining chain not provided yet 5

Handling & Genotyping Instructions
Name Type

Mammalian Phenotype Ontologies
 

References
Name Type Comment
Erlandsson et al., 2001 J Exp Med 194(5):557-70 Webblink Joining chain-expressing and -nonexpressing B cell populations in the mouse.

Author's Comments
 
Serum IgM and IgG reduced 6-8 fold, while serum IgA elevated 14-fold in JDTA mice. Mice are immune competent although the serum Ig response compared with WT mice was reduced 6-fold at day 14 but only 4-fold at day 45 after immunization.